Interspecific and intraspecific identification of Dendrobium based on the psbAtrnH intergenic region sequences and the 5S rRNA gene spacer sequences北大核心CSCD

This research aimed to investigate the interspecific and intraspecific identification of Dendrobium by using the multi-locus method so as to provide a molecular basis for Dendrobium identification through the combination of chloroplast psbA-trnH intergenic region sequences and ribosome 5S rRNA gene spacer sequences. PCR direct sequencing was applied to detect the chloroplast psbA-trnH intergenic region sequences as well as the ribosome 5S rRNA gene spacer sequences of 12 Dendrobium species, while the psbA-trnH intergenic region sequences of Dendrobium denneanum dq-2 variety and dq- 5line were cloned and sequenced for single nucleotide polymorphism (SNP) analyzing. The sequences were analyzed by the software Sequencher4.14, Bioedit7.0, MEGA5.2 and Dansp5.0; the interspecific and intraspecific Kimara-2-Parameter(K2P) distances were also calculated. The phylogenetic tree (using Neighbor joining method) was constructed with Bulbophyllum odoratissimum and Bletilla striata as outgroup. The results showed an average length of chloroplast psbA-trnH gene sequences in Dendrobium as 742.3 bp, with 72 variable sites, including 33 information sites; the average length of the ribosome 5S rRNA gene spacer sequences in Dendrobium was 336.4 bp, with 213 variable sites including 139 information sites. Using psbAtrnH intergenic region sequences in combination with ribosome 5S rRNA gene spacer sequences can not only identify D. denneanum, D. hancockil, D. thysiflorum, D. devonianum, D. moniliforme, D. chrysotoxum, D. officinale, D. heterocarpum and D. nobile, but also differentiate D. officinale from different geographical populations, and distinguish the dq-2 variety and dq 5line with SNP in the multi locus of D. denneanum.     

基于GPS的北京市2007-2012年地面沉降变化研究

利用经验模态分解(EMD)方法处理2007-2012年的北京GPS数据,并通过与地下水埋深比较分析近年来的地面沉降。首先通过2007-2012年的年沉降速率,发现北京沉降中心连片,形成了中东部的沉降漏斗。DSQI累积沉降量达到510 mm,DSQI、CHAO、NLSH三站点的年沉降速率分别为85 mm/a,41.7 mm/a和20mm/a。其次采用EMD方法提取DSQI、NLSH的垂向序列的趋势项,分析其沉降趋势。最后,对地下水埋深和GPS站点沉降趋势之间的关系进行了对比分析,地面沉降变化趋势与地下水水位变化具有较好的一致性,地下水水位变化是地面沉降发生发展的主要诱因。     

The impact of humanitarian context conditions and individual characteristics on aid worker retention

High employee turnover rates constitute a major challenge to effective aid provision. This study examines how features of humanitarian work and aid workers’ individual characteristics affect retention within one humanitarian organisation, Médecins Sans Frontières (MSF) Holland. The study extends existing research by providing new theoretical explanations of employment opportunities and constraints and by engaging in the first large‐scale quantitative analysis of aid worker retention. Using a database of field staff (N=1,955), a logistic regression is performed of the likelihood of reenlistment after a first mission. The findings demonstrate that only 40 per cent of employees reenlist for a second mission with MSF Holland, and that workplace location and security situation, age, and gender have no significant effect. Individuals are less likely to reenlist if they returned early from the first mission for a personal reason, are in a relationship, are medical doctors, or if they come from highly developed countries. The paper reflects on the findings in the light of policy.     

贵州镇远提钒尾渣固体废弃物二次资源利用研究

为了开发无尾矿、少尾矿提钒工艺,对贵州镇远江古钒矿实验室扩大试验流程产生的尾矿进行资源化利用研究,尾渣物质组成研究表明:提钒尾渣的主要化学成分为二氧化硅,主要矿物为石英,尾渣放射性检测结果高于建筑物主体墙材中放射性指标的要求,不能直接应用于建筑主体材料.经用尾渣烧成水泥试验结果表明:尾渣烧成的水泥熟料基本化学成分、物理性能测试结果符合GB/T 21372-2008硅酸盐水泥熟料要求.